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Structural analysis of polysaccharides from mushroom hericium Erinaceus mftccb 134
Bleha, R. ; Čopíková, J. ; Sushytskyi, Leonid ; Vannucci, Luca ; Sinica, A. ; Jozífek, M. ; Jablonský, I. ; Klouček, P.
The aim of this research was the structural characterisation of polysaccharides isolated from fruiting bodies of the cultivating medicinal mushroom Hericium erinaceus MFTCCB 134. Cold and hot water extracts (CWE, HWE) were obtained and purified, and followed by alkali extraction from the reminder solids. The composition and structure of the fractions were analysed by FTIR spectroscopy, and neutral sugars/linkage analyses were determined by GC/FID and GC/MS, respectively. Galactose, fucose and glucose were found in purified CWE,\nwhile glucose predominated in purified HWE.
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Active preparatives based on koji fermentation and medical mushrooms extracts
Živná, Kristýna ; Dzurická, Lucia (referee) ; Hoová, Julie (advisor)
This bachelor's thesis deals with the optimization of the growth of the rare species fungus of the hedgehog mushroom on cereal substrates for the purpose of the subsequent to cultivation of lactic acid bacteria. The theoretical part is focused on the description of the rare fungus species and the use of the cereals as substrate and the characterization of their active substances. It also deals with lactic fermentation and its use. In the experimental part, the preparation of the cereal substrate for the cultivation of the hedgehog mushroom was optimized. The method of inoculation of the cereals substrate with liquid mycelium in combination with plate mycelium was chosen as the most applicable. In this combination, the substrate does not dry out and grows evenly from both sides of the substrate. The composition of the cereal substrate with grown mycelium was characterized (antioxidant activity, carbohydrates, proteins). Finally, the substrate was inoculated with lactic bacteria. The lactic bacteria added nutritional value to the product. Fermentation products (antioxidant activity, carbohydrates, proteins) were characterized, and bacterial viability was analysed.
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Substrate processing for cultivation of Hericium erinaceus with different supplements and heat treatments
Sobotová, Radmila ; Jablonský, Ivan (advisor) ; David, David (referee)
The aim of the thesis was to determine if the substrate treatment with temperature lower than 100°C could replace the sterilization at 120°C. Another task was to compare different types of substrates and supplements.
For the substrate treatment were selected temperatures of 90°C for 48 hours, 90°C for 20 hours, 121°C for 2 hours, 80°C for 20 hours and 70°C for 24 hours. The treatment with temperature lower than 100°C could replace the sterilization at 120°C.
In the experiments were used substrates compound of beech sawdust or straw pellets supplemented by wheat bran or wheat groats. The research was also focused to influence of substrate fermentation on growth characteristics of Hericium erinaceus. The most suitable substrate for mycelium growth and subsequent fructification of Hericium erinaceus were beech sawdust. Faster mycelial growth and higher yield of fruiting body were achieved in the case of supplemented substrate of sawdust beech by wheat bran or wheat groats. There were observed no effects of fermentation substrates on mycelium growth of Hericium erinaceus.
Another part of the research was dedicated to mycelium growth and fructification of Hericium coralloides. The best substrate for Hericium coralloides is beech sawdust without supplements.
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